Recombinant:
CCK-8 Assay:Article Title: Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor
Article Snippet: .. The cells were treated with complete DMEM medium containing eATP at different concentrations (0, 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mM) for 24 h. Another group of cells was treated with complete DMEM medium containing P2X7R competitive antagonist (A804598, MCE) at different concentrations (0, 2.5, 5, 10, 20, 50, 100 μM) for 24 h. Subsequently, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The absorbance at 450 nm was measured using a microplate reader (Prolong, China) to calculate cell metabolic viability, and the appropriate concentration was selected for subsequent experiments. ..
Article Title: Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor.
Article Snippet: .. The cells were treated with complete DMEM medium containing eATP at different concentrations (0, 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mM) for 24 h. Another group of cells was treated with complete DMEM medium containing P2X7R competitive antagonist (A804598, MCE) at different concentrations (0, 2.5, 5, 10, 20, 50, 100 μM) for 24 h. Subsequently, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The absorbance at 450 nm was measured using a microplate reader (Prolong, China) to calculate cell metabolic viability, and the appropriate concentration was selected for subsequent experiments. ..
Incubation:Article Title: Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor
Article Snippet: .. The cells were treated with complete DMEM medium containing eATP at different concentrations (0, 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mM) for 24 h. Another group of cells was treated with complete DMEM medium containing P2X7R competitive antagonist (A804598, MCE) at different concentrations (0, 2.5, 5, 10, 20, 50, 100 μM) for 24 h. Subsequently, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The absorbance at 450 nm was measured using a microplate reader (Prolong, China) to calculate cell metabolic viability, and the appropriate concentration was selected for subsequent experiments. ..
Article Title: Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor.
Article Snippet: .. The cells were treated with complete DMEM medium containing eATP at different concentrations (0, 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mM) for 24 h. Another group of cells was treated with complete DMEM medium containing P2X7R competitive antagonist (A804598, MCE) at different concentrations (0, 2.5, 5, 10, 20, 50, 100 μM) for 24 h. Subsequently, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The absorbance at 450 nm was measured using a microplate reader (Prolong, China) to calculate cell metabolic viability, and the appropriate concentration was selected for subsequent experiments. ..
Concentration Assay:Article Title: Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor
Article Snippet: .. The cells were treated with complete DMEM medium containing eATP at different concentrations (0, 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mM) for 24 h. Another group of cells was treated with complete DMEM medium containing P2X7R competitive antagonist (A804598, MCE) at different concentrations (0, 2.5, 5, 10, 20, 50, 100 μM) for 24 h. Subsequently, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The absorbance at 450 nm was measured using a microplate reader (Prolong, China) to calculate cell metabolic viability, and the appropriate concentration was selected for subsequent experiments. ..
Article Title: Extracellular ATP induces the proinflammatory phenotype transition of MH7A cells by activating P2X7 receptor.
Article Snippet: .. The cells were treated with complete DMEM medium containing eATP at different concentrations (0, 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mM) for 24 h. Another group of cells was treated with complete DMEM medium containing P2X7R competitive antagonist (A804598, MCE) at different concentrations (0, 2.5, 5, 10, 20, 50, 100 μM) for 24 h. Subsequently, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The absorbance at 450 nm was measured using a microplate reader (Prolong, China) to calculate cell metabolic viability, and the appropriate concentration was selected for subsequent experiments. ..
Clinical Proteomics:Article Title: m6A‐Modified KLF11 Inhibits Macrophage Foam Cell Formation by Transcriptionally Suppressing ATP5B Expression in Atherosclerosis
Article Snippet: Macrophage foam cell formation is the mark of atherosclerosis (AS).. Krüppellike factor (KLF)11 prevented the development of AS in diabetic conditions.. However, it is not clear whether KLF11 inhibits macrophage foam cell formation.
other:Article Title: Arginine synthesis pathway and ASS1 play a critical role in mRNA translation reprogramming and ICI resistance in cutaneous melanoma
Article Snippet: YUMM1.1 and YUMM1.7 knocked-down for ASS1 were maintained in DMEM medium supplemented with L-argininosuccinic acid lithium salt 100μg.mL -1 (Sigma Aldrich). lZl-methyl- DL -aspartic acid (MDLA) was purchased from MedChem Express and was dissolved in PBS and the pH adjusted with NaOH to obtain a pH≈7.4.
Whole Genome Amplification:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
Live Cell Imaging:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
BIA-KA:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
SDS Page:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
Enzyme-linked Immunosorbent Assay:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
Activity Assay:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
AST Assay:Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
Article Snippet: Recombinant human insulin (Yeasen, 40112ES60) and exendin-4 (MCE, HY-13443) were used as model therapeutics. .. Streptozotocin (KKL, KM6241), FITC (Solarbio, F8070), FITC-dextran (Beyotime, ST2930, ST2935, ST2940, ST2947), pepsin (Sigma-Aldrich, P7012), pancreatin (Sigma-Aldrich, P7545), sodium alginate (Sigma-Aldrich, 180947), Rhodamine-Wheat Germ Agglutinin (WGA) (Maokangbio, MP6326), DMEM medium (Keygentec, KGL1206-500), FBS (HyClone, SV30087.03), Antifade medium containing DAPI (Beyotime, P0131), Fluo-4 AM (Beyotime, S1060), Dil (Beyotime, C10360 ), DiBAC 4 (3) (MCE, HY-101892), Live-cell imaging solution (Thermo, A59688DJ), BCA assay kit (Beyotime, P0009), SDS-PAGE gel (Genscript, M42015), PVDF membranes (Millipore, IPVH00010), Insulin ELISA kit (Elabscience, E-EL-H2665), Exendin-4 ELISA kit (Byabscience, BY- EM221897 ), Glutamic-pyruvic Transaminase Activity Assay Kit (Solarbio, BC1555), Aspartate Aminotransferase Activity Assay Kit (Solarbio, BC6435), Urea Nitrogen Content Assay Kit (Solarbio, BC1535). .. β-lactoglobulin solution (20 mg/mL in ultrapure water) was acidified to pH 2.0 with HCl and subjected to thermal fibrillization at 90 °C for 5 h. The resulting nanofibrils were length-reduced through pulsed tip sonication (SCIENTZ-IID JY98; 60 W, 1 s on/1 s off cycles for 15 min) and subsequently purified via dialysis (100 kDa MWCO, Spectrum Laboratories) against acidified ultrapure water.
Purification:
Cell Culture:
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